DNA grafting and arrangement on oxide surfaces for self-assembly of Al and CuO nanoparticles

Théo Calais (1) , David Bourrier (2) , Aurélien Bancaud (3) , Yves J. Chabal (4) , Alain Estève (1) , Carole Rossi (1)

1 LAAS-NEO - Équipe Nano-ingénierie et intégration des oxydes métalliques et de leurs interfaces
2 LAAS-TEAM - Service Techniques et Équipements Appliqués à la Microélectronique
3 LAAS-MILE - Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement
4 UT Dallas - University of Texas at Dallas [Richardson]

DNA-directed assembly of nano-objects as a means to manufacture advanced nanomaterial architectures has been the subject of many studies. However, most applications have dealt with noble metals as there are fundamental difficulties to work with other materials. In this work, we propose a generic and systematic approach for functionalizing and characterizing oxide surfaces with single-stranded DNA oligonucleotides. This protocol is applied to aluminum and copper oxide nanoparticles due to their great interest for the fabrication of highly energetic heterogeneous nanocomposites. The surface densities of streptavidin and biotinylated DNA oligonucleotides are precisely quantified combining atomic absorption spectroscopy with conventional dynamic light scattering and fluorimetry, and maximized to provide a basis for understanding the grafting mechanism. First, the streptavidin coverage is consistently below 20% of the total surface for both nanoparticles. Second, direct and unspecific grafting of DNA single strands onto Al and CuO nanoparticles largely dominates the overall functionalization process:

Domaines: Chimie

Théo Calais, David Bourrier, Aurélien Bancaud, Yves J. Chabal, Alain Estève, et al.. DNA grafting and arrangement on oxide surfaces for self-assembly of Al and CuO nanoparticles. Langmuir, 2017, 33 (43), pp.12193-12203. ⟨10.1021/acs.langmuir.7b02159⟩. ⟨hal-01613194⟩